Produção de Amphiprion ocellaris (Cuvier, 1830) alimentados com farinha de linhaça (Linum usitatissimum L.) em diferentes concentrações

Abstract

This study aimed to verify whether isoproteic diets with different percentages of linseed meal (LM), a functional food, influence the reproductive activity the ornamental marine fish A. ocellaris, in closed recirculation system. It was conducted from May to November/2011. There followed a completely randomized design with five treatments and four replications. We monitored physical-chemical parameters of water (temperature, pH, dissolved oxygen, alkalinity, salinity, total ammonia, nitrite). Were observed the secondary sexual characteristics and registrated length, weight, number of nests and eggs. The inclusion levels of linseed by treatment were: 0% (control), 5%, 15%, 25% and 35%.These means were compared by Tukey test (5%), Dunnet test (5%) and parameters by Student´s test t (α=0,05). The physical and chemical parameters of the cultivation water did not differs (p> 0.05) meeting the needs of fishes and micro-organisms. In the biological filter was reduced total ammonia in undetectable levels and nitrite in 36.63%. There was no occurrence of opportunistic pathogenic bacteria such as Pseudomonas and Aeromonas, but Edwardsiella gender was present at the beginning of cultivation, without demonstrations. Sterilizing with ultraviolet light kept control of mesophilic heterotrophic bacteria and inactivation of pathogens. Spawning occurred in treatments with 5% and 15% of LM. The allometry was negative (b<3), for both sexes, with the addition of growth in weight. The mean relative condition factor (Kn) for females (p> 0.05) indicating welfare in fish farming. The percentage of linseed meal 5% has potential for use as a food that can influence reproductive activities of clownfish, requiring further studies with longer trial.