Leveduras associadas a moluscos bivalves e água do Estuário do Rio Subaé, São Francisco do Conde, BA

Abstract

This study reports the isolation and molecular identification of yeasts isolated from water samples and bivalve molluscs (Mytella guyanensis and Crassostrea rhizophorae) commonly collected in the estuary of the river Subaé, São Francisco do Conde, Bahia. In addition to the central theme of isolation and identification of water yeast and the two above bivalve molluscs are discussed the implications to health and the environment, since the basin Subaé Rio suffers anthropic action, at some points, contemplating the estuarine environment, becoming an important factor for environmental monitoring. The isolates were identified in the study of the domains D1 / D2 gene ribosomal RNA (rRNA) 26S and, for some samples was conducted further study of RNA polymerase II gene region elucidating the identification to the species level. Additionally phylogenetic analysis of five species were carried out, which had not been previously reported in bivalve molluscs. 70 isolates were identified in yeast, distributed in nine genera, and Candida spp. and Rhodotorula spp. the prevalent genres. The study also revealed predominance of Filo Ascomycota with 48 representatives across the Basidiomycota with 17 isolates. Candida haemulonii, Candida maris, Candida infanticola, Candida natalensis, Hanseniaspora opuntiae had their first reported presence bivalve mollusks. This is the first report related to the molecular analysis of the yeast community in bivalve molluscs in northeastern Brazil. This study extends the knowledge of the diversity of bivalves associated with yeasts that are widely consumed form of raw or lightly cooked, can act as a further indicator of the quality of these fish.