Semente de linhaça na dieta de coelhos e a relação da qualidade do sêmen fresco e resfriado com o perfil metabólico

Abstract

The study aimed to evaluate the effect of flaxseed inclusion (Linum usitatissimum) in the rabbits diet, through physical and morphological quality of fresh semen and post-cooling and metabolic profile. We used 20 male New Zealand, divided into four groups have been fed whit levels of flaxseed in total diet: 0; 3; 6 and 9%, for a period of 100 days. The seminal collection were initiated 15 days after initiation of the diets and were performed by the technique of artificial vagina, using as a female manequim at the frequency of once a week. After collection, proceeded to review physical and morphological seminal. The results were diluted in Tris-yolk medium, subjected to cooling to 5°C and evaluated at time 0, 2, 12, 24, 48 and 72h after cooling for motility, sperm vigor and morphology. The animals underwent blood sampling every 22 days to determine the metabolic profile. Data were assessed by regression analysis at 5% significance. The inclusion of flaxseed in the diet of rabbits did not alter the aspect seminal, with predominant white color and watery consistency. There was no difference in the physical and morphological aspects of fresh semen. There was no difference in sperm motility and vigor at 0, 2, 12, 24, 48 and 72h post-cooling. There was quadratic positive for plasma glucose and total cholesterol. There was no difference in plasma triglycerides, LDL-cholesterol, VLDL-cholesterol and urea. There was cubic behavior for HDL-cholesterol and creatinine. The inclusion of up to 9% of flaxseed in the diet of rabbits does not alter the physical and morphological parameters of fresh semen and cooled. However, there were changes in blood metabolites of animals.