Cultivo in vitro de Plantas Medicinais:Ocimum basilicum L. e Cissus sicyoides L.

Abstract

The objective of this study was to induce in vitro multiplication of Ocimum basilicum L., and achieving a protocol the callus in Cissus sicyoides L. in vitro. In in vitro organogenesis of Ocimum basilicum L., were used as explants, segments internodais plants in vitro. These were cultured in MT medium and BAP: 0.0, 2.0 and 4.0 mg L -1 . It was evaluated: percentage of responsive explants and number of buds / explant. For rooting, shoots were transferred to MT medium containing 0.5 g L -1 of activated charcoal and NAA concentrations: 0.0, 1.0, 2.0 and 3.0 mg L-1 . It was evaluated: percentage of rooting, length of the longest root (cm), weight of fresh matter (g) and dry matter (g). The addition of 2.0 mg L -1 BAP promoted the best organogenic response. With roots, does not require the addition of NAA. For the callus of Cissus sicyoides L., were used as explants, leaf segments of plants from the field, after disinfection were inoculated into MT medium + 1.0 mg L-1 NAA. We evaluated the % of explants % of survivors and contamination. For the cultivation using the MT medium + 1.0 mg L-1 NAA and BAP: 2.0, 4.0, 6.0 and 12.0 mg L -1 . It was evaluated, the number of compact and friable callus. For the first and second subculture the material was introduced into MT medium + 1.0 mg L-1 NAA was the same varying concentrations of BAP, and assessed the number of friable callus formed and the % of area covered with calluses. The amount of callus formed was obtained over the subcultures through the number of repeats formed during the same, and also obtained, the fresh matter weight (g) and dry (g). Then there was the testing phytochemicals. The disinfection was shown to be ineffective. For the callus of Cissus sicyoides L. from leaf segment it is necessary the addition of 6.0 mg L-1 BAP to the medium of culture. It was the presence of cardiac heterosides in callus of Cissus sicyoides L.