Desenho de primers ssr (simple sequence repeat) para regiões genômicas do tomateiro (Solanaceae) visando a transferibilidade para Physalis angulata L. (Solanaceae)

Abstract

The present study aimed to develop SSR (simple sequence repeat) molecular markers from the tomato genome (Solanum lycopersicum), a species belonging to the solanaceae family, aiming at its possible application in Physalis angulata, a species well known for its benefits. food, medicinal and commercial products. With the help of bioinformatics tools from a database it was possible to develop microsatellite markers from the sequenced Tomato genome, these sequences are deposited in the National Center for Biotechnology Information (NCBI) GenBank, from which they were drawn primes in the Websat application. In all, 1000 SSR marker modules were generated, which had their quality assessed by the NetPrimer application. Among these markers, 120 primers classified as optimal within the parameters used were selected. Among the analyzed microsatellite regions of tomato, there was a predominance of replications: dinucleotides 43.26%, with predominant AT / TA motifs in 79.91%; trinucleotides with 36.77% and hexanucleotides with 11.60%. The primers designed had a mean concentration of CG% of 46.32%, classified as excellent in the literature. This study reinforces the importance of using bioinformatics tools in the search of already sequenced genomes, emphasizing among other applications the development of markers, besides being an alternative for genetic studies of species whose genome has not yet been sequenced, allowing a possibility of transferability between genomes related species thus contributing to the study of their genetic variability.