Diversidade de isolados de Trichoderma do clado Harzianum da restinga de Guaibim, BA

Abstract

In general, studies on the biological diversity of restingas are limited to fauna and flora and knowledge about the microbial diversity of this ecosystem is limited to few studies on mycorrhizal fungi and yeasts. Due to anthropic actions, taxonomic groups are being lost without even knowing their biotechnological importance. The objective of this work was to study the genetic diversity of 32 isolates of fungi of the genus Trichoderma collected in the periodically flooded forest and thicket of the restinga of Guaibim - BA, and to study the phylogeny of these fungi. The genetic diversity study using the BOX A 1R primer showed the existence of 12 distinct genetic profiles among the 32 Trichoderma isolates analyzed. The 12 remaining profiles were represented by one isolate per profile. These isolates were distributed in distinct groups in the genetic similarity dendrogram obtained by the UPGMA clustering method and Jaccard coefficient from the BOX-PCR genetic profiles. For molecular phylogeny, barcode gene tef-1 (Elongation factor 1 alpha) and rpb2 (second largest subunit of RNA polymerase) were sequenced and compared with orthologous genes of types species deposited in the National Center for Biotechnology Information (NCBI) database.). The molecular identification of 12 isolates revealed that they are related to species of the Clade Harzianum, close to the isolates Trichoderma breve and Trichoderma zelobreve and represent at least 3 new species.