Estudo da variabilidade genética do vírus do amarelo letal (“papaya lethal yellowing virus”, plyv) e do vírus da meleira do mamoeiro, (“papaya meleira virus”, pmev)

Abstract

Papaya (Carica papaya L.) is a fruit of great social and economic importance to Brazil. The viruses are among the diseases that cause the greatest damage to the crop. The “Papaya lethal yellowing virus” (PLYV) and “Papaya meleira virus” (PMeV), despite the damages to production, there is little information on their genomes and genetic variability. The objective of this work was to generate information on genetic variability between different isolates of PLYV and PMeV. For PLYV, we performed the sequencing of a fragment comprising the 3 'region of the viral replicase and the 5' region of the coat protein, and for PMeV the fragment comprised a portion of the viral replicase. The analysis of PLYV nucleotide sequences showed high identity with Sobemovirus species, while PMeV showed no identity with any plant virus previolsly described. Comparisons of the deduced amino acid sequences amongPLYV isolates revealed low genetic variability, while for PMeV was observed a greater variability.