Eficiência simbiótica de bactérias diazotróficas isoladas de solos com histórico de deposição de manipueira em feijão-caupi cultivar epace 10

Abstract

The objective of this work was to evaluate the symbiotic efficiency of diazotrophic bacteria isolated from soils with a history of manure deposition on germination, nodulation and development of cowpea, EPACE 10 cultivar. The experiment was conducted in a completely randomized design, in the greenhouse of the Center of Agrarian, Environmental and Biological Sciences - CCAAB, UFRB, with 12 treatments and four replicates, using two authorized spores for culture, INPA 3-11B and UFLA 3-84, a strain in test phase UFLA 3-164, seven strains isolated from soils with historic of deposit of cassava wastewater, UFBB NA32B1, UFRB NA84B1-2, UFRB NA52A2, UFRB NA31A2, UFRB NA41A2-2, UFRB NA51C2 and UFRB NA31A1; And two controls, with no source of N and one with mineral N in the EPACE 10 cowpea cultivar. After sowing, emergence and germination were evaluated during the first 10 days up to the V2 stage at the 15th (V2 stage) 105.3 mg of nitrogen was added to the control with mineral N, and after 50 days of planting (R2 stage) the plants were collected to evaluate the variables: chlorophyll index, leaf number, height, number of nodules, matter Fresh and dry nodules and shoot dry matter, root dry matter, total dry matter, efficiency and effectiveness. The germination of cowpea was not influenced by the inoculation of diazotrophic bacteria. The strains UFRB NA52A2, UFRB NA51C2, UFRB NA31A1 and UFLA 3-164, reduced the emergence percentage of cowpea. The selected strains of soils with a history of manure tree deposition are able to establish symbiosis with cowpea. Efficacy and efficiency were not interfered with by inoculation of the strains. The strains UFRB NA32B1, UFRB NA41A2-2 are capable of stimulating the root development of cowpea, EPACE 10 cultivar.