Aplicação de uma mPCR para detecção e identificação de microbactérias diretamente de lesões bovinas suspeitas de tuberculose provenientes de matadouro-frigorífico sob inspeção estadual no Estado da Bahia

Abstract

Bovine tuberculosis diagnosis has been widely studied by molecular assays. These methods have enabled greater agility, high sensitivity and specificity. Furthermore allow differential diagnosis between diseases and accurate identification of the etiologic agent which is of great importance to the epidemiology, treatment and prognosis especially in public health. The aim of this study was to associate as additional resource to the post-mortem examination in a slaughterhouse, a multiplex PCR performed directly from the cheesy material collected in two slaughterhouses under inspection in Bahia state. Seventy-seven samples of cattle were analyzed. In Southwest sixty-one suspect cattle lymph nodes were analyze, resulting in 4.92% positive samples in isolation and 18% in mPCR for M. bovis. In Reconcavo 13 cattle suspected samples were analyze resulting in 61.54% positive isolation with no significant statistical difference in the molecular assay where was detected 54% of M. bovis. For both, the mPCR is viable attesting significant advantage to disclose results within a maximum period of 48 hours, ability to detect multiple species in a single reaction, not depend on bacterial culture and does not require the living micro-organism to make positive the diagnosis. DNA showed good concentration on the extraction and appropriate purity. The results indicate that the protocol was effective, fast and very promising in slaughterhouse vigilance.