Sincronização de ovulação com redução de dose hormonal aplicada no acuponto bai hui em ovinos e caprinos

Abstract

The aim of this study was to evaluate the effect of 30% of the dose of equine chorionicgonadotropin (eCG) and prostaglandin F (PGF) in Bai Hui 2α2αacupoint (BH) in ovulation synchronization protocols for goats and sheep. Thirty sheep and forty goat were used and received intravaginal sponges with 60mg of ® medroxyprogesterone acetate (MAP) (Progespon , Syntex, Argentina) for 7 days, ® followed by applying: group (G) 1: 0.5mL (132.5μg) of cloprostenol (Ciosin ), a ® synthetic analogue of PGF, and 300UI eCG (Folligon ), both intramuscular (IM), 2αG2: 0.15mL (39.75µg) of PGF on BH acupoint and 300UI eCG IM; G3: 0.5mL 2α intramuscularly and PGF 90UI eCG at BH; G4: 0.15mL of PGF and 90UI eCG, 2α2αboth in BH and G5: 0.15mL of PGFand 90UI eCG, both at false acupoint (IM). 2α Data were evaluated by analysis of variance at 5% probability and chi -square test. There was no statistical difference for any of the parameters evaluated (p>0.05) (sheep and goats respectively): percentage of animals in estrus (93.32% and 95%); interval from sponge removal to onset of estrus (48.16 ± 6.03h and 49.72 ± 8.93h), interval from sponge removal to the end of estrus (69.68 ± 5.43h and 76.84 ± 11.98h), length of estrus (24.00 ± 4.74h and 27.08 ± 8.68h), follicular growth rate (0.85 ± 0.10 mm / day and 0.86 ± 0.29 mm / day); range sponge removal ovulation(73.76 ± 5.91 h and 78.28 ± 10.82 h); range of onset of estrus to ovulation (26.56 ± 2.36 h and 28.52 ± 5.44 h), size of the largest follicle (6.7 ± 0.4mm and 6.8 ± 4.4mm), number of ovulations (1.39 ± 0.16 and 1.32 ± 0.23), plasma progesterone concentration (9.60 ± 2.06ng/mL and 10.28 ± 1.65ng/mL) and the cost of synchronization protocol per animal was US$13.35, US$$ 12.37, US$7.47, US$6.49 and US$6.49 for G1, G2 , G3, G4 and G5, respectively. The use of progestagen associated with reduced doses of eCG and PGF applied at Bai Hui 2αacupoint or false acupoint was effective in synchronizing ovulation in goats and sheep.