Caracterização molecular de acessos do banco ativo de germoplasma de citros da Embrapa Mandioca e Fruticultura

Abstract

The Active Germplasm Bank of Citrus, located at Embrapa Mandioca e Fruticultura, Cruz das Almas-BA, contains about 800 accessions of Citrus and related species, many from unknown ancestry. The level of genetic diversity present in this collection so far remains unknown. This study aimed to characterize, through the ISSR markers, 83 accessions from the Citrus Germplasm Bank in order to determine the level and organization of genetic diversity in the collection. In order to do so, total genomic DNA was extracted, quantified and genotyped by using 9 ISSR primers. A total of 47 markers were identified, from which 39 were polymorphic (82.98%) and monomorphic 8 (17.02%). The computer programs GENE and MEGA-4 were used to generate a dissimilarity matrix and a dendrogram, respectively, revealing the formation of five major groups among the accessions. The greatest dissimilarity was observed among accessions Ilhoa Goiânia and Oneco, provenient from the species C. sinensis and C. reticulata, respectively. However, for a more complete characterization of these genotypes it is necessary to identify a larger number of ISSR.