Dissimilaridade genética em cinco cultivares de Ricinus communis L. através de marcadores RAPD

Abstract

Castor beean is an oilseed of great economic importance. This plant in Brazil is a typical plant from the semi-arid region, whis represents 90% Northeast of the planted area. The great variability shown by this crop is seen in botanical and agronomic characteristics and can be also evaluated by DNA polymorphism, with the use of molecular markers such as RAPD. The technique of DNA markers, known as RAPD, has been used to estimate genetic diversity by increasing the efficiency in breeding programs. Thus, this study aimed to RAPD markers for assessing the genetic dissimilary vergence among five cultivars of castor beans. Ofel DNA was extracted and quantified from Ricinus communis L. Leaf tissue a total of twenty random primers were used for genotiping cultivars. Since it is a dominant marker, bands were by (0) given (1) for presence a 1.5% agarose gel. We a total of 202 markers (74 polymorphic (36.6%) and 128 monomorphic (63.4%) bands). Werk The OPD 05 primer generated 18 bands, the largest number among the initiators used, and the primer OPD 03, only 5. The genetic cliss between cultivars was calculated using the Jaccard's coefficient UPGMA clustering method and the GENE software. A dendrogram was generated by the MEGA4 Program. Chemical analysis identificd the formation of two groups: using Group I, formed by cultivars EBDA MPA 17, MPA 18 EBDA, EBDA EBDA and MPA 26 MPA 31 and group II consisting of 11 cultivars EBDA MPA. Cultivars EBDA EBDA MPA MPA 11 and 26 were the most dissimilaridad. Thus, it is observed that there is among cultivars. Promising combinations are expected between cultivars EBDA EBDA MPA 11 and MPA 26. Molecular markers such as RAPD are effective to characterize the genetic variability among castor genotypes.