Características biológicas da geoprópolis da abelha social sem ferrão Uruçu (Melipona scutellaris Latreille, 1811) proveniente da Baía do Iguape-Ba

Abstract

This study had since objective valued the production of geoprópolis of Melipona scutellaris at the Bail of the Iguape, Bahia as well as his microbiological composition and his antimicrobial activity against Staphylococcus aureus and Salmonella Enteritidis. For this reason the work was divided into three stages. In the first step were initially installed two Meliponários in Bay of Iguape, using ten colonies of M. scutellaris. During twelve months, the bees were stimulated to produce geoprópolis using the method collector device geoprópolis. Weekly to geoprópolis produced was collected and replaced. The influence of climatic parameters on the productive potential of geoprópolis M. scutellaris was verified. The production of geoprópolis by M. scutellaris during the study period, is correlated with the variable radiation, wind direction and wind gust. In the second stage evaluation was performed on the microbiological quantification front the presence of contaminating microorganisms, according to the seasons, using 24 samples of geoprópolis collected within one year. Therefore, it was determined: aerobic mesophilic, psychrotrophic aerobic bacteria, molds and yeasts, Clostridium sulphite reducing, total coliforms, Escherichia coli, presence and absence for Salmonella sp. and coagulase positive Staphylococcus. Geoprópolis has an average maximum microbial count of 1010 UFC.g-1, however, absence sulphite reducing Clostridium and Salmonella sp. In the third step we evaluated the antimicrobial activity of the hydroalcoholic extract of geoprópolis M. scutellaris front of Gram-positive and Gram-negative bacteria. Was determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. The hydroalcoholic extract of geoprópolis, presented antimicrobial activity against the growth of microorganism S. aureus and Salmonella Enteritidis.