Relação de parentesco de híbridos trifoliolados de citros utilizando parâmetros de homozigosidade via marcadores SSR e mitocondriais

Abstract

Citriculture has great economic importance in Brazil and in the world, contributing to the strengthening of the economy. Citrus rootstocks (PEs) are largely responsible for this success, as the Copa x rootstock combination forms the basis of orchards and is responsible for high productivity. Embrapa Cassava and Fruitculture, over the last 30 years, has observed a field of crosses where PEs were selected for presenting desirable characteristics such as large number of seeds and resistance / tolerance to major biotic and abiotic factors, whose relationship is undetermined. Therefore, the main objectives of the present work are: To determine the kinship of selected PEs and to determine the level of homozygosity between the PE varieties: Sunki Common, Sunki Tropical and Sunki Wonder. This study will help to assess the degree of kinship among seven EPs and their likely parents to serve as registration information with the Ministry of Agriculture, Livestock and Supply. A total of 26 citrus genotypes were used and divided into three groups. The polymorphism between rootstocks and their possible parents was analyzed using SSRs (Simple Sequence Repeats) and mitochondrial markers using PCR technique, where the reactions were electrophoresed using 3% agarose gel. Genotyping was performed following the pattern of absence and presence of bands, and later molecular analyzes performed using statistical programs such as GENES, Power Marker and R. Although genotypes are very similar to the genetic material, it was possible to determine the assumptions. parenting for PEs. Therefore, to be sure of the origin of the parents, it is necessary to synthesize new primers, so that a larger number of regions of the genome were amplified.