Estabelecimento de protocolo de regeneração por embriogênese somática e transformação genética do mamoeiro

Abstract

This study aimed to: I- to develop a protocol for regeneration by somatic embryogenesis (ES) of papaya Carica papaya L.), fruit species native from tropical America; II-establish a curve selection with the herbicide glufosinate ammonia (GA) and III-embryogenic cells transformed by biolistic method. Therefore, immature zygotic embryos were used for the establishment of ES, and embryogenic cells were used in the curve selection with GA for future selection of transformed embryos. Embryogenic cells were bombarded using the plasmid vector pBI426, these underwent histochemical assay using the reagent X-Gluc. The plantlets regenerated of papaya are in the stadium rooting; curve for selection with GA, concentrations above 5 mg / L inhibited the growth of embryogenic cells. The biolistic method was efficient by histochemical assay. From these results it can be concluded that the proposed protocols have been efficient in the induction of ES and by biolistic gene transformation.