Eficiência e caracterização morfofisiológica de bactérias oriundas de nódulos de feijão-caupi (Vigna unguiculata L.) em solos de Santo Amaro BA

Abstract

The cowpea is an important food legume for being rich source of vitamins and proteins. For sustainable crop production one of the most viable technologies both from an environmental point of view as economic is the use of inoculants for biological nitrogen fixation. This study aimed to carry out isolation, cultural characterization and authentication of isolates of bacteria present in cowpea plant roots grown in soil contaminated by lead and treated with sisal waste. For the isolation and characterization of diazotrophs nodules were randomly assigned to the root system of the plants. There were isolated 61 bacterial strains of nodes. Cultural characteristics evaluated for each isolated were: the growth rate measured by isolated colonies of onset time; the average diameter of isolated colonies; the change of pH; form of board; elevation of the board; the consistency of the colony; the indicator of absorption; light transmission and color of the colonies. 61 were obtained isolated in pure culture, of which 49 showed rapid growth and 12 intermediate; and the modification of the pH of the culture medium 22 and 39 basified acidified. Isolated intermediate and alkaline growth UFRB C4, UFRB C8A , UFRB C11B , C16B UFRB , UFRB C18 , UFRB C20B , UFRB C26 , UFRB C27 , UFRB C36 and UFRB C39 have potential for further studies of selection of strains for cultivation in soil Recôncavo of Bahia.